Roma are missing defenders Marco Rui, Thomas Vermaelen and Abdullah Nura ahead of the home tie on Thursday through injury; whilst the hosts are also without Alessandro Florenzi (cruciate ligament) and Francesco Totti (hip injury).
Head coach Luciano Spalletti is likely to recall midfielder Mohamed Salah to the line-up, who was rested during the win over Austria Vienna earlier this month.
Meanwhile, the visitors travel to Rome with centre-back Marian Cisovsky the only injury worry for Plzen who is suffering from a virus infection.
High-scoring Roma return to Europa League action this week, when they welcome Czech side Viktoria at the Stadio Olimpico on Thursday night.
The Giallarossi need just one win in their final two group matches to secure a place into the Round of 32, who sit top on eight points and remain the only unbeaten side in Group E with two wins and two draws.
Luciano Spalletti’s men have scored 11 goals in their last three European meetings, including an emphatic 4-2 away win to Austria Vienna earlier in the month.
Roma will be overwhelming favourites for victory, having yet to lose in their last seven home games this season, averaging over three goals per game at the Olimpico.
On the other hand, Viktoria Plzen make the trip to the Italian capital two points off second following their 1-1 draw against Astra.
Currently, it leaves them bottom of the group on three points, and they’ll be massive underdogs to come away with anything in Rome who are winless in their last nine matches.
In the reverse fixture back in September, both sides shared the spoils during a 1-1 draw in Romania, with Marek Bakos cancelling out a Diego Perotti penalty.
Alisson (GK), Peres, Jesus, Fazio, De Rossi, Nainggolan, Leandro Paredes, Iturbe, Salah, Dzeko, Perotti
Lung (GK), Sapunaru, Alves, Fabrizio, Maranhao, Felipe Teixeira, Budescu, Florin, Boudjemaa, Niculae, Alibec